The "i-Vice Ganda Mo 'Ko Challenge" featuring Nanay Rosario is a playful and heartwarming segment where Vice Ganda engages with his mother. They participate in various fun challenges that test their knowledge of each other, filled with humor and lighthearted banter. The segment showcases their close relationship, filled with laughter, affectionate teasing, and relatable family dynamics. Overall, it highlights the joy of family connections while reflecting Vice Ganda's signature comedic style.